Blunted Indomethacin-Induced Downregulation of Aquaporins by Nitric Oxide Synthesis Inhibition in Rats
The Korean Journal of Physiology and Pharmacology
; : 213-216, 2006.
Article
en En
| WPRIM
| ID: wpr-728555
Biblioteca responsable:
WPRO
ABSTRACT
The present study was aimed to determine whether nitric oxide (NO) plays a role in the regulation of aquaporin (AQP) channels in the kidney. Male Brattleboro rats (250~300 g body weight) were used. The experimental group was treated with N(G)-nitro-L-arginine methyl ester (L-NAME, 100 mg/L drinking water) for 1 week, and cotreated with indomethacin (5 mg/kg, twice a day, i.p.) for the last two days. Control groups were treated with either L-NAME for 1 week, indomethacin for 2 days, or without any drug treatment. The abundance of AQP1, AQP2 and AQP3 proteins in the kidney was determined by Western blot analysis. Indomethacin downregulated AQP channels, whereas L-NAME by itself showed no significant effects on them. The indomethacin-induced downregulation of AQP2 and AQP3 was significantly blunted in L-NAME-treated rats, while that of AQP1 was not affected. These results suggest that endogenous NO, when stimulated, may downregulate AQP channels that are specifically regulated by AVP/cAMP pathway in the kidney.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Ratas Brattleboro
/
Regulación hacia Abajo
/
Indometacina
/
Western Blotting
/
NG-Nitroarginina Metil Éster
/
Acuaporinas
/
Ingestión de Líquidos
/
Acuaporina 3
/
Riñón
/
Óxido Nítrico
Límite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2006
Tipo del documento:
Article