Characterization of Ionic Currents in Human Neural Stem Cells
The Korean Journal of Physiology and Pharmacology
;
: 131-135, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-728596
ABSTRACT
The profile of membrane currents was investigated in differentiated neuronal cells derived from human neural stem cells (hNSCs) that were obtained from aborted fetal cortex. Whole-cell voltage clamp recording revealed at least 4 different currents a tetrodotoxin (TTX)-sensitive Na+ current, a hyperpolarization-activated inward current, and A-type and delayed rectifier-type K+ outward currents. Both types of K+ outward currents were blocked by either 5 mM tetraethylammonium (TEA) or 5 mM 4-aminopyridine (4-AP). The hyperpolarization-activated current resembled the classical K+ inward current in that it exhibited a voltage-dependent block in the presence of external Ba2+ (30micrometer) or Cs+ (3micrometer). However, the reversal potentials did not match well with the predicted K+ equilibrium potentials, suggesting that it was not a classical K+ inward rectifier current. The other Na+ inward current resembled the classical Na+ current observed in pharmacological studies. The expression of these channels may contribute to generation and repolarization of action potential and might be regarded as functional markers for hNSCs-derived neurons.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tetrodotoxina
/
Potenciales de Acción
/
4-Aminopiridina
/
Tetraetilamonio
/
Células-Madre Neurales
/
Membranas
/
Neuronas
Límite:
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2008
Tipo del documento:
Artículo
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