Potentiation of Innate Immunity by beta-Glucans
Mycobiology
;
: 144-148, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-729476
ABSTRACT
beta-Glucans have been known to exhibit antitumor activities by potentiating host immunity by an unknown mechanism. The C-type lectin dectin-1, a beta-glucan receptor, is found on the macrophage and can recognize various beta-glucans. Previously, we demonstrated the presence of beta-glucan receptor, dectin-1, on the Raw 264.7 cells as well as on murine mucosal organs, such as the thymus, the lung, and the spleen. In order to investigate immunopotentiation of innate immunity by beta-glucan, we stimulated a murine macrophage Raw 264.7 cell line with beta-glucans from Pleurotus ostreatus, Saccharomyces cerevisiae, and Laminaria digitata. Then, we analyzed cytokines such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 by reverse transcription-polymerase chain reaction (RT-PCR). In addition we analyzed gene expression patterns in beta-glucan-treated Raw 264.7 cells by applying total mRNA to cDNA microarray to investigate the expression of 7,000 known genes. When stimulated with beta-glucans, the macrophage cells increased TNF-alpha expression. When co-stimulation of the cells with beta-glucan and lipopolysaccharide (LPS), a synergy effect was observed by increased TNF-alpha expression. In IL-6 expression, any of the beta-glucans tested could not induce IL-6 expression by itself. However, when co-stimulation occurred with beta-glucan and LPS, the cells showed strong synergistic effects by increased IL-6 expression. Chip analysis showed that beta-glucan of P. ostreatus increased gene expressions of immunomodulating gene families such as kinases, lectin associated genes and TNF-related genes in the macrophage cell line. Induction of TNF receptor expression by FACS analysis was synergized only when co-stimulated with beta-glucan and LPS, not with beta-glucan alone. From these data, beta-glucan increased expressions of immunomodulating genes and showed synergistic effect with LPS.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfotransferasas
/
Polisacáridos
/
Saccharomyces cerevisiae
/
Bazo
/
Timo
/
ARN Mensajero
/
Receptores Inmunológicos
/
Expresión Génica
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Línea Celular
/
Citocinas
Límite:
Humanos
Idioma:
Inglés
Revista:
Mycobiology
Año:
2010
Tipo del documento:
Artículo
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