Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis
Mycobiology
;
: 108-112, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-729537
ABSTRACT
To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1 + null mutant and 4 proteins successfully identified glycolipid anchored surface precursor, beta-glucosidase (Psu1), cell surface protein, glucan 1,3-beta-glucosidase (Bgl2), and exo-1,3 beta-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfotransferasas
/
Schizosaccharomyces
/
Resinas Acrílicas
/
Proteínas
/
Pared Celular
/
Beta-Glucosidasa
/
Proteoma
/
Bases de Datos de Proteínas
/
Glucano 1,3-beta-Glucosidasa
/
Electroforesis en Gel de Poliacrilamida
Idioma:
Inglés
Revista:
Mycobiology
Año:
2010
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS