Development of Detection Methods for Cellulolytic Activity of Auricularia auricula-judae
Mycobiology
;
: 74-77, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-729543
ABSTRACT
To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from 15~35degrees C. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at 25degrees C for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fenolsulfonftaleína
/
Bencenosulfonatos
/
Carbono
/
Celulosa
/
Rojo Congo
/
Diminazeno
/
Concentración de Iones de Hidrógeno
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Mycobiology
Año:
2010
Tipo del documento:
Artículo
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