Screening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains
Mycobiology
;
: 70-73, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-729544
ABSTRACT
Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over 30degrees C. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from 30degrees C to 37degrees C. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Saccharomyces cerevisiae
/
Levaduras
/
ADN
/
Secuencia de Bases
/
Biblioteca de Genes
/
Ciclo Celular
/
Tamizaje Masivo
/
Pleurotus
/
Agaricales
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Inglés
Revista:
Mycobiology
Año:
2010
Tipo del documento:
Artículo
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