Cloning and Sequence Analysis of the Cellobiohydrolase I Genes from Some Basidiomycetes
Mycobiology
;
: 107-110, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-729685
ABSTRACT
Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. The DNA sequences obtained were similar showing high identity to the cbhI gene family. These cbhI genes were partial consisting of three coding regions and two introns. The deduced amino acid sequences exhibited significant similarity to those of fungal CBHI enzymes belonging to glycosyl hydrolase family 7.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Basidiomycota
/
Bromuros
/
ADN
/
Secuencia de Bases
/
Intrones
/
Celulasa
/
Reacción en Cadena de la Polimerasa
/
Secuencia de Aminoácidos
/
Células Clonales
/
Análisis de Secuencia
Límite:
Humanos
Idioma:
Inglés
Revista:
Mycobiology
Año:
2012
Tipo del documento:
Artículo
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