Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green
Mycobiology
;
: 258-262, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-729695
ABSTRACT
cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of H2O2 improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to 150 microM within 90 min.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Compuestos Organometálicos
/
Oxidorreductasas
/
Peroxidasas
/
Pichia
/
Colorantes de Rosanilina
/
ADN Complementario
/
Peroxidasa
/
Phanerochaete
/
Oxidorreductasas de Alcohol
/
Glioxal
Idioma:
Inglés
Revista:
Mycobiology
Año:
2012
Tipo del documento:
Artículo
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