Identification of the Genes Involved in the Fruiting Body Production and Cordycepin Formation of Cordyceps militaris Fungus
Mycobiology
;
: 37-42, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-729863
ABSTRACT
A mutant library of Cordyceps militaris was constructed by improved Agrobacterium tumefaciens-mediated transformation and screened for degradation features. Six mutants with altered characters in in vitro and in vivo fruiting body production, and cordycepin formation were found to contain a single copy T-DNA. T-DNA flanking sequences of these mutants were identified by thermal asymmetric interlaced-PCR approach. ATP-dependent helicase, cytochrome oxidase subunit I and ubiquitin-like activating enzyme were involved in in vitro fruiting body production, serine/threonine phosphatase involved in in vivo fruiting body production, while glucose-methanol-choline oxidoreductase and telomerase reverse transcriptase involved in cordycepin formation. These genes were analyzed by bioinformatics methods, and their molecular function and biology process were speculated by Gene Ontology (GO) analysis. The results provided useful information for the control of culture degeneration in commercial production of C. militaris.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Biología
/
Agrobacterium tumefaciens
/
Complejo IV de Transporte de Electrones
/
Telomerasa
/
Biología Computacional
/
Cordyceps
/
Agrobacterium
/
Ontología de Genes
/
Frutas
/
Hongos
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Mycobiology
Año:
2015
Tipo del documento:
Artículo
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