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Production of Blastospore of Entomopathogenic Beauveria bassiana in a Submerged Batch Culture
Mycobiology ; : 218-224, 2009.
Artículo en Inglés | WPRIM | ID: wpr-729924
ABSTRACT
The principal objective of this study was to determine the optimal liquid culture conditions in shake flasks for maximal sporulation of Beauveria bassiana. The optimal initial pH for the spore production of B. bassiana using Potato Dextrose Broth was 5.2. The screening in shake flasks of carbon and nitrogen sources resulted in the identification of an optimal medium based on 3% sucrose and 1% casamino acid, with a C N ratio of 22 4. Using this medium, a production level of 5.65 x 107 spores per ml was obtained after 5 days of culture. Using 3% corn meal, 2% corn steep powder, and 2% rice bran, the maximum spore concentration of 8.54 x 108/ml was achieved 8 days after inoculation at 25degrees C in a rotary shaking incubator operated at 200 rpm. This represents a yield gain of approximately 2.89 times that of pre-optimization.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Esporas / Sacarosa / Solanum tuberosum / Carbono / Tamizaje Masivo / Zea mays / Beauveria / Técnicas de Cultivo Celular por Lotes / Comidas / Glucosa Tipo de estudio: Estudio de tamizaje Idioma: Inglés Revista: Mycobiology Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Esporas / Sacarosa / Solanum tuberosum / Carbono / Tamizaje Masivo / Zea mays / Beauveria / Técnicas de Cultivo Celular por Lotes / Comidas / Glucosa Tipo de estudio: Estudio de tamizaje Idioma: Inglés Revista: Mycobiology Año: 2009 Tipo del documento: Artículo