Interaction of Mesenchymal Stem Cells and Osteoblasts for in vitro Osteogenesis
Yonsei Medical Journal
;
: 187-197, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-73210
ABSTRACT
It has recently been reported that bone marrow-derived mesenchymal stem cells (MSCs), which are systemically administrated to different species, undergo site-specific differentiation. This suggests that the tissue specific cells may cause or promote the differentiation of the MSCs toward their cell type via a cell-to-cell interaction that is mediated not only by hormones and cytokines, but also by direct cell-to-cell contact. In this study, in order to assess the possible synergistic interactions for osteogenesis between the two types of cells, the MSCs derived from rabbit bone marrow were co-cultured with rat calvarial osteoblasts in direct cell-to-cell contact in a control medium (CM) and in an osteogenic medium (OM). The cell number, alkaline phosphatase activity, and amount of calcium deposition were assayed in the cultures of MSCs, osteoblasts, and co-cultures of them in either OM or CM for up to 40 days. The cell numbers and the alkaline phosphatase activities in the co-culture were somewhere in between those of the osteoblast cultures and the MSC cultures. The amounts of deposited calcium were lower in the co-culture compared to those of the other cultures. This suggests that there are little synergistic interactions during osteogenesis in vitro between the rat osteoblasts and rabbit MSCs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoblastos
/
Osteogénesis
/
Células Madre
/
Calcificación Fisiológica
/
Comunicación Celular
/
Recuento de Células
/
Diferenciación Celular
/
División Celular
/
Fosfatasa Alcalina
/
Mesodermo
Límite:
Animales
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2003
Tipo del documento:
Artículo
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