Identification and function analysis of a novel transcript 630 in acute T-cell lymphoblastic leukemia Jurkat cell lines / 中华实用儿科临床杂志

ABSTRACT

Objective To identify and analyze a novel transcript 630 in acute T-cell lymphoblastic leukemia cell (T-ALL) Jurkat cell lines.Methods RNA-sequencing was applied to transcriptome profiling.The novel transcript 630 was identified by reverse transcription polymerase chain reaction (RT-PCR) combined with sequencing.The expression level of the novel transcript was detected in some tumor cell lines and normal cells.The sequences for small interfering RNA (siRNA) against 630 were designed and synthesized.Then,the effects of siRNA were determined by real-time PCR.Apoptotic cells and cell cycle analysis were investigated with the flow cytometry after siRNA-mediated knockdown of 630.Results The novel transcript 630 was identified.High expression level of 630 was detected in Jurkat E6-1,HL60 and human umbilical vein endothelial(HUVE) cells.The knockdown of transcript 630 induced a decrease of cellular proliferation.Conclusions The novel transcript 630 is one of the novel transcripts in Jurkat cells.The decreased expression level of transcript 630 can suppress the proliferation of leukemia cells.The novel transcript 630 might contribute to the treatment of T-ALL.