Relationship among levels of peripheral blood miRNA-21 and TGF-β1,CCL5 in patients with acute myocardial infarction and its significance / 心血管康复医学杂志

ABSTRACT

Objective:To observe levels of peripheral blood miRNA-21,transforming growth factor β1 (TGF-β1) and CC type chemokine ligand 5 (CCL5) in patients with acute myocardial infarction (AMI),and explore their relation-ship and clinical significance.Methods:A total of 57 AMI patients treated in our hospital were selected as AMI group.Another 50 healthy people undergoing physical examination in our hospital simultaneously were selected as healthy control group.Peripheral blood miRNA-21 expression,levels of CCL5,TGF-β1,creatine kinase (CK),CK isoenzyme MB (CK-MB) and cardiac troponin I (cTnI),and coronary Gensini score were measured in all subjects.Relationship among above indexes was analyzed in AMI patients.Results:Compared with healthy control group,there were significant rise in miRNA-21 expression [ (0.67 ± 0.06) vs.(0.89 ± 0.09)],levels of CCL5 [ (17.53 ± 3.75) ng/ml vs.(35.37 ± 5.06) ng/ml],CK [ (85.24 ± 18.33) U/L vs.(173.29 ± 32.56) U/L],CK-MB [ (16.37 ± 2.03) U/L vs.(23.34 ± 2.51) U/L],cTnI [ (11.25 ± 3.23) ng/ml vs.(31.45 ± 8.79) ng/ml] and Gensini score [ (1.65 ± 0.26) scores vs.(43.25 ± 12.13) scores],and significant reduction in TGF-β1 level [ (31.45 ± 8.79) ng/ml vs.(11.25 ± 3.23) ng/ml] in AMI group,P＝0.001 all;in AMI group,peripheral blood miRNA-21 expression was significant positively correlated with levels of CCL5,CK,CK-MB and cTnI (r＝0.476～0.593,P＜0.05 all),and significant inversely correlated with TGF-β1 level and coronary Gensini score (r＝ -0.417,-0.625,P＜0.05 both).Conclusion:Peripheral blood miRNA-21 expression is high,and closely associated with levels of CCL5,TGF-β1 and coronary stenotic degree in AMI patients.It may be used as a potential target for clinical diagnosis and treat-ment of AMI.