Identification of CD44 splice variant in Korean colorectal cancers and cell lines
Journal of Korean Medical Science
;
: 169-175, 1995.
Artículo
en Inglés
| WPRIM
| ID: wpr-7338
ABSTRACT
CD44 is a glycoprotein expressed in a wide variety of cell types. Recently expression of some alternatively-spliced variants of CD44 transcripts (CD44v) has been suggested to play a potential role in tumor metastasis and the detection of CD44v containing exon 6 to 11 may be helpful for the diagnosis of cancers. Expressions of CD44v containing exon 6 to 11 were investigated in 20 human colorectal cancer samples, peripheral blood leukocytes isolated from colorectal cancer patients, and 4 colorectal cancer cell lines using reverse transcription-polymerase chain reaction and Southern blot analysis. The standard form of CD44 transcripts was expressed in all samples tested. CD44v containing exon 6 to 11 was expressed in 18 cases of colorectal cancers (sensitivity = 90%), 3 out of 4 cell lines, and one normal tissue (specificity = 95%). These results suggest that the expression of CD44v containing exon 6 to 11 can be regarded as tumor specific and that this marker may be helpful for the early diagnosis of colon cancers, if specimens from the early stage are available.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Datos de Secuencia Molecular
/
Células Tumorales Cultivadas
/
Secuencia de Bases
/
Neoplasias Colorrectales
/
Adenocarcinoma
/
Biomarcadores de Tumor
/
Regulación Neoplásica de la Expresión Génica
/
Empalme del ARN
/
Southern Blotting
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
/
Estudio de tamizaje
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Journal of Korean Medical Science
Año:
1995
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS