Effects of high-fat diet on liver function and intestinal bacterial community in rats / 中华临床营养杂志

ABSTRACT

Objective To investigate the influence of high-fat diet on liver function and intestinal bacte-rial community through building rat models. Methods 20 rats of 21 days old were divided into two groups ran-domly as normal diet group fed with standard chow diet and high-fat group fed with high-fat diet. After 6 weeks, feces of rats in both groups were obtained for 16S rRNA high-through sequencing of the intestinal bacterial com-munity. Results After 6 weeks high-fat diet, total protein (TP) (55. 79±3. 75, P=0. 002), globin (GLB) ( 34. 9±2. 53, P<0. 001), albumin (ALB) /GLB (. 60±0. 02, P<0. 001), alkaline phosphatase (ALP) (373. 80±63. 05, P<0. 001), total cholesterol (TC) (1. 94±0. 23, P<0. 001), low density lipoprotein (LDL) (0. 76±0. 93, P<0. 001), LDL/high density lipoprotein (HDL) (1. 43±0. 22, P<0. 001), and tri-glyceride (TG) (1. 48±0. 50, P=0. 015) increased compared with the normal diet group. Additionally, intes-tinal bacterial diversity and evenness decreased significantly. The dominant bacteria were Bacteroidetes, Firmi-cutes, and Proteobacteria, with averaged relative abundances as 56. 36%, 35. 31%, and 6. 61%, respectively. The relative abundances of Bacteroidetes deceased (P=0. 007), those of Firmicutes increased (P=0. 020), and those of Proteobacteria were kept stable (P=0. 928) after a 6-week high-fat diet. Furthermore, the intesti-nal bacterial community structure changed distinctly between the two groups by 16s rRNA high-through sequen-cing. Conclusion High-fat diet can lead to change of intestinal bacterial community structure and further result in liver function damnification as well as obesity.