Molecular Cloning of hSC2 Encoding 5α-reductase-like Protein
Annals of Dermatology
;
: 252-258, 2000.
Artículo
en Inglés
| WPRIM
| ID: wpr-73468
ABSTRACT
BACKGROUND:
The human homologue of the SC2 gene from a human dermal papilla cell cDNA library has been isolated and designated hSC2. HSC2 protein also shares similarity with 5 -reductase, a protein important in testosterone metabolism.OBJECTIVE:
Prior to knowing the functions of hSC2 in dermal papilla, we cloned it and analyzed its relative expression levels in adult tissues and cancer cell lines.METHODS:
hSC2 was isolated from low abundant clones in dermal papilla cDNA library using cDNA array hibridization method. Full-length clone was sequenced and we studied its expression in different tissues by Northern blot hybridization.RESULTS:
Sequence data reveals a single open reading frame, encoding a putative hydrophobic protein with a calculated molecular weight of 36 kDa. Its deduced amino acid sequences are almost 97.4% identical to t4ose of rat protein. Northern blot hybridization shows that hSC2 cDNA recognizes a 1.35 kb transcript that was expressed in various epithelial and mesenchymal tissues including testis and liver.CONCLUSION:
We have cloned and analysed tissue distributions of hSC2. It was interesting that it had homology with 5α-reductase isozymes. Further studies will be needed to understand the involvement of hSC2 in androgen hormone signaling.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Testículo
/
Testosterona
/
Distribución Tisular
/
Biblioteca de Genes
/
Línea Celular
/
Northern Blotting
/
Sistemas de Lectura Abierta
/
Secuencia de Aminoácidos
/
Células Clonales
/
Clonación Molecular
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Annals of Dermatology
Año:
2000
Tipo del documento:
Artículo
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