Effect of intracellular-free calcium changes on the process of pancreatic cancer cell line apoptosis induced by As2O3 / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 422-424, 2001.
Artículo
en Chino
| WPRIM
| ID: wpr-735393
ABSTRACT
Objective:
To study the effect of intracellular-free calcium and the expression of Fas and Fas L on the process of pancreatic carcinoma cell apoptosis.Methods:
Apoptosis induced by 2 μmol/L arsenic trioxide in pancreatic cancer cell lines SW-1990 was investigated.Concentration of intracellular-free calcium ([Ca2+]i) was determined by Fura-2a fluorescein load technique. Fas and FasL were determined by flow cytometry.Results:
Pancreatic cancer cells treated with 2 μmol/L arsenic trioxide presented apoptotic features intact cell membrane, chromatin condensation, nucleic fragmentation and apoptotic body formation; agarose electrophoresis showed marked ladder; flow cytometery analysis showed a sub-G1 cell peak. In the process of pancreatic carcinoma cell apoptosis Fas and FasL and the [Ca2+]i were significantly higher than that in the control.Conclusion:
The pancreatic cancer cell apoptosis induced by arsenic trioxide is related to Fas and FasL expression by the cancer cells and the [Ca2+]i increase in the cancer cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Academic Journal of Second Military Medical University
Año:
2001
Tipo del documento:
Artículo
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