Expression of Human Interferon-λ1 and Interferon-ε Gene in WI-38 Cells and Comparison of Their Biological Activity / 中国生物工程杂志
China Biotechnology
;
(12): 24-33, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-735624
ABSTRACT
The biological activities i. e. antineoplastic activities and antiviral activity of the two novel kinds of interferons hIFN-λ1 and hIFN-ε were studied and compared. First the fusion expression vector pcDNA3.1A-hIFN-λ1-His and pcDNA3.1A-hIFN-ε-His by PCR was constructed, then the two kinds of plasmids were transfected into WI-38 (human embryonic lung cells ) with liposome. And cytopathic effect (CPE) suppression test was used to study and compare the antiviral activities of rhIFNλ1-His and rhIFN-ε-His, meanwhile MTT assay was used to detect their antineoplastic activities. It was found that, antiproliferative activity and MxA protein induction shown by rhIFN-λ1-His is more powerful than of rhIFN-ε -His. The antiviral molecular mechanisms of both hIFN-λ1 and hIFN-ε are related to MxA. The foundation for further study on the bioactivities and mechanism of action of hIFN-λ1 and hIFN-ε was established.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Biotechnology
Año:
2007
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS