Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation
Clinical and Experimental Otorhinolaryngology
; : 86-94, 2019.
Article
en En
| WPRIM
| ID: wpr-739226
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVES: The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques. METHODS: Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up. RESULTS: DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycosaminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dL and 53 mg/L). CONCLUSION: Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time.
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Texto completo:
1
Índice:
WPRIM
Asunto principal:
Tráquea
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ADN
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Ácido Láctico
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Ingeniería de Tejidos
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Ácido Desoxicólico
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Liofilización
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Glucosa
Límite:
Animals
Idioma:
En
Revista:
Clinical and Experimental Otorhinolaryngology
Año:
2019
Tipo del documento:
Article