PD-L1 Expression on Circulating CD34+ Hematopoietic Stem Cells Closely Correlated with T-cell Apoptosis in Chronic Hepatitis C Infected Patients
International Journal of Stem Cells
;
: 78-86, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-739919
ABSTRACT
BACKGROUND AND OBJECTIVES:
Lack of understanding of the interplay between hematopoietic stem cells (HSCs) and the immune system has severely hampered stem cell research. Programmed death-1 (PD-L1) has been reported on parenchymal cells in patients with chronically inflamed livers and found to play an essential role in T cell homeostasis regulation. However, the bidirectional interaction between HSCs and lymphocytes remains elusive. Here, we aimed to get more insight into circulating CD34+ HSCs PD-L1 expression and T cell apoptosis in chronic HCV infected patients.METHODS:
CD34+ HSCs were isolated and purified by immunomagnetic separation. PD-L1 expression was analyzed by quantitative PCR and flow cytometry. Furthermore, co-culture experiments between CD34+ HSCs and T-lymphocytes were established. T-cell lymphocyte apoptosis in peripheral blood and in cultures was detected.RESULTS:
CD34+ HSCs constitutively express low levels of PD-L1. Its expression is up-regulated in chronic HCV infected patients. Moreover, PD-L1 expression on circulating CD34+ HSCs enhanced T cell apoptosis in peripheral blood and co-culture.CONCLUSION:
Our results suggest novel bidirectional interplay between HSCs and lymphocytes mediated by PD-L1 expression on CD34+ HSCs. PD-L1 expression correlated with T-cell lymphocyte apoptosis. This may contribute to immunomodulatory properties of HSCs which improves its use for allogeneic transplantation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Trasplante Homólogo
/
Células Madre Hematopoyéticas
/
Linfocitos
/
Linfocitos T
/
Reacción en Cadena de la Polimerasa
/
Apoptosis
/
Separación Inmunomagnética
/
Técnicas de Cocultivo
/
Hepatitis C Crónica
/
Investigación con Células Madre
Límite:
Humanos
Idioma:
Inglés
Revista:
International Journal of Stem Cells
Año:
2018
Tipo del documento:
Artículo
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