Pja2 Inhibits Wnt/β-catenin Signaling by Reducing the Level of TCF/LEF1
International Journal of Stem Cells
;
: 242-247, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-739922
ABSTRACT
Ubiquitination of proteins plays an essential role in various cellular processes, including protein degradation, DNA repair, and cell signaling pathways. Previous studies have shown that protein ubiquitination is implicated in regulating pluripotency as well as fate determination of stem cells. To identify how protein ubiquitination affects differentiation of embryonic stem cells, we analyzed microarray data, which are available in the public domain, of E3 ligases and deubiquitinases whose levels changed during stem cell differentiation. Expression of pja2, a member of the RING-type E3 ligase family, was up-regulated during differentiation of stem cells. Wnt/β-catenin signaling is one of the most important signaling pathways for regulation of the self-renewal and differentiation of embryonic stem cells. Pja2 was shown to bind to TCF/LEF1, which are transcriptional factors for Wnt/β-catenin signaling, and regulate protein levels by ubiquitination, leading to down-regulation of Wnt signaling activity. Based on these results, we suggest that E3 ligase Pja2 regulates stem cell differentiation by controlling the level of TCF/LEF1 by ubiquitination.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Madre
/
Regulación hacia Abajo
/
Sector Público
/
Ubiquitina
/
Ubiquitina-Proteína Ligasas
/
Reparación del ADN
/
Células Madre Embrionarias
/
Ubiquitinación
/
Proteolisis
/
Proteasas Ubiquitina-Específicas
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
International Journal of Stem Cells
Año:
2018
Tipo del documento:
Artículo
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