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Antibody-based Screening of Porphyromonas gingivalis Proteins Specifically Produced in Patients with Chronic Periodontitis
International Journal of Oral Biology ; : 201-207, 2018.
Artículo en Inglés | WPRIM | ID: wpr-740083
ABSTRACT
Porphyromonas gingivalis is among the major etiological pathogens of chronic periodontitis. The virulence mechanisms of P. gingivalis is yet to be identified as its activity is largely unknown in actual disease process. The purpose of this study is to identify antigens of P. gingivalis expressed only in patients with chronic periodontitis using a unique immunoscreening technique. Change Mediated Antigen Technology (CMAT), an antibody-based screening technique, was used to identify virulence-associated proteins of P. gingivalis that are expressed only during infection stage in patients having chronic periodontitis. Out of 13,000 recombinant clones screened, 22 tested positive for reproducible reactivity with rabbit hyperimmune anti-sera prepared against dental plaque samples acquired from periodontitis patients. The DNA sequences of these 18 genes were determined. CMAT-identified protein antigens of P. gingivalis included proteins involved in energy metabolism and biosynthesis, heme and iron binding, drug resistance, specific enzyme activities, and unknown functions. Further analysis of these genes could result in a novel insight into the virulence mechanisms of P. gingivalis.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Periodontitis / Virulencia / Resistencia a Medicamentos / Secuencia de Bases / Tamizaje Masivo / Células Clonales / Porphyromonas / Porphyromonas gingivalis / Factores de Virulencia / Placa Dental Tipo de estudio: Estudio diagnóstico / Estudio pronóstico / Estudio de tamizaje Límite: Humanos Idioma: Inglés Revista: International Journal of Oral Biology Año: 2018 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Periodontitis / Virulencia / Resistencia a Medicamentos / Secuencia de Bases / Tamizaje Masivo / Células Clonales / Porphyromonas / Porphyromonas gingivalis / Factores de Virulencia / Placa Dental Tipo de estudio: Estudio diagnóstico / Estudio pronóstico / Estudio de tamizaje Límite: Humanos Idioma: Inglés Revista: International Journal of Oral Biology Año: 2018 Tipo del documento: Artículo