Lignans with NADPH Oxidase 2 (NOX2)-inhibitory Activity from the Fruits of Schisandra chinensis
Natural Product Sciences
; : 59-65, 2018.
Article
en En
| WPRIM
| ID: wpr-741595
Biblioteca responsable:
WPRO
ABSTRACT
An isoform of NADPH oxidase (NOX), NOX2 is a superoxide-generating enzyme involved in diverse pathophysiological events. Although its potential as a therapeutic target has been validated, there is no clinically available inhibitor. Herein, NOX2-inhibitory activity was screened with the constituents isolated from Schisandra chinensis, which has been reported to have antioxidant and reactive oxygen species (ROS)-scavenging effects. Among the partitions prepared from crude methanolic extract, a chloroform-soluble partition showed the highest NOX2-inhibitory activity in PLB-985 cell-based NOX2 assay. A total of twenty nine compounds (1 – 29) were identified from the chloroform fraction, including two first isolated compounds; dimethyl-malate (25) and 2-(2-hydroxyacetyl) furan (27) from this plants. Of these constituents, two compounds (gomisin T, and pregomisin) exhibited an NOX2-inhibitory effect with the IC₅₀ of 9.4 ± 3.6, and 62.9 ± 11.3 µM, respectively. They are confirmed not to be nonspecific superoxide scavengers in a counter assay using a xanthine-xanthine oxidase system. These findings suggest the potential application of gomisin T (6) and other constituents of S. chinensis to inhibit NOX2.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Oxidorreductasas
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Cloroformo
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Especies Reactivas de Oxígeno
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Lignanos
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Superóxidos
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NADPH Oxidasas
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Schisandra
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Metanol
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Frutas
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NADP
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Natural Product Sciences
Año:
2018
Tipo del documento:
Article