Identification and HPLC Quantification of a Phytoecdysone and Three Phenolic Glycosides in Lamium takesimense Nakai
Natural Product Sciences
; : 241-246, 2018.
Article
en En
| WPRIM
| ID: wpr-741635
Biblioteca responsable:
WPRO
ABSTRACT
The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 µm, 4.6 mm × 250 mm) with a gradient elution of H₂O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order: lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g).
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Plantas
/
Rutina
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Cromatografía Líquida de Alta Presión
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Ácido Acético
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Lamiaceae
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Fenol
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Ecdisona
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Ecdisterona
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Glicósidos
/
Métodos
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Natural Product Sciences
Año:
2018
Tipo del documento:
Article