Study on the Marker Steroids of New Zealand Deer (Cervus elaphus var. scoticus) Velvet Antler by UPLC-MS/MS and HPLC-PDA Methods
Natural Product Sciences
; : 49-58, 2019.
Article
en En
| WPRIM
| ID: wpr-741644
Biblioteca responsable:
WPRO
ABSTRACT
Eleven steroid hormones (SHs: androstene-3,17-dione, estrone, β-estradiol, α-estradiol, testosterone, dehydroepiandrosterone, 17á-hydroxyprogesterone, medroxyprogesterone, megestrol acetate, progesterone, and androsterone) were detected from New Zealand deer (Cervus elaphus var. scoticus) velvet antler (NZA, 鹿茸). A method for the quantification of eleven SHs was established by using ultraperformance liquid chromatography (UPLC)-MS/MS. The linearities (R² > 0.991), limits of quantification (LOQ values, 0.3 ng/mL to 23.1 ng/mL), intraday and interday precisions (relative standard deviation: RSD 0.999), LOQ values (30 ng/mL to 350 ng/mL), intraday and interday precisions (RSD < 1.93%), and recovery rates (97.2% to 103.5%) for the three 7-O-CSs were determined. These quantitative methods are accurate, precise, and reproducible. As a result, it is suggested that the five steroid compounds of androstene-3,17-dione, androsterone, 7-ketocholesterol, 7α-hydroxycholesterol, and 7β-hydroxycholesterol could be marker steroids of NZA. These methods can be applied to quantify or standardize the marker steroids present in NZA.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Cuernos de Venado
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Progesterona
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Esteroides
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Testosterona
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Ciervos
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Cromatografía Liquida
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Deshidroepiandrosterona
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Acetato de Megestrol
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Estrona
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Androsterona
Límite:
Animals
País/Región como asunto:
Oceania
Idioma:
En
Revista:
Natural Product Sciences
Año:
2019
Tipo del documento:
Article