Effects of atorvastatin on expression of lipid metabolism-related genes in HepG2 cells / 吉林大学学报(医学版)

ABSTRACT

Objective:To explore the effects of atorvastatin on the natural antisense transcription of apolipoprotein A1 (apoA1-NAT) in the HepG2cells and its influence in the expressions of lipid metabolism related genes.Methods:The HepG2cells were intervened with different concentrations (0, 1, 10and 100nmol·L-1) of atorvastatin, and the 0nmol·L-1 atorvastatin group was used as control group.The total RNA of HepG2cells in various groups were extracted at different time points (6, 12, 24, and 48h) .The mRNA expression levels of lipid metabolism-related genes and apoA1-NAT expression levels were detected by Real-Time PCR method.Results:The morphology of HepG2 cells in 1and 10nmol·L-1 atorvastain groups was normal.Compared with 6h, the expression levels of apoA1-NAT in the HepG2cells in 10nmol·L-1 atorvastatin group at 12, 24and 48hwere significantly decreased (P＜0.01) in a time-dependent manner.Under the same condition, the expression level of apoA1mRNA in the HepG2cells at 48hwas increased significantly compared with 6h (P＜0.01) .Compared with control group, the expression levels of low density lipoprotein receptor (LDLR) in the HepG2 cells in100nmol·L-1 atorvastatin group, scavenger receptor-class B type 1 (SRB1) in 10nmol·L-1 atorvastatin group and ATP binding cassette transporter A1 (ABCA1) in 1and 10nmol·L-1 atorvastatin groups were increased (P＜0.01) in different degrees.Conclusion:Atorvastatin can promote the expressions of lipid metabolism-related genes by inhibiting the expression of apoA1-NAT, and promote the process of reverse cholesterol transport.