Overexpression of Plasminogen Activator Inhibitor-1 in Advanced Gastric Cancer with Aggressive Lymph Node Metastasis / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 718-726, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-74292
ABSTRACT
PURPOSE:
The purpose of this study is to investigate differentially expressed genes using DNA microarray between advanced gastric cancer (AGC) with aggressive lymph node (LN) metastasis and that with a more advanced tumor stage but without LN metastasis. MATERIALS ANDMETHODS:
Five sample pairs of gastric cancer tissue and normal gastric mucosa were taken from three patients with T3N3 stage (highN) and two with T4N0 stage (lowN). Data from triplicate DNA microarray experiments were analyzed, and candidate genes were identified using a volcano plot that showed > or = 2-fold differential expression and were significant by Welch's t test (p < 0.05) between highN and lowN. Those selected genes were validated independently by reverse-transcriptase-polymerase chain reaction (RT-PCR) using five AGC patients, and tissue-microarray (TMA) comprising 47 AGC patients.RESULTS:
CFTR, LAMC2, SERPINE2, F2R, MMP7, FN1, TIMP1, plasminogen activator inhibitor-1 (PAI-1), ITGB8, SDS, and TMPRSS4 were commonly up-regulated over 2-fold in highN. REG3A, CD24, ITLN1, and WBP5 were commonly down-regulated over 2-fold in lowN. Among these genes, overexpression of PAI-1 was validated by RT-PCR, and TMA showed 16.7% (7/42) PAI-1 expression in T3N3, but none (0/5) in T4N0 (p=0.393).CONCLUSION:
DNA microarray analysis and validation by RT-PCR and TMA showed that overexpression of PAI-1 is related to aggressive LN metastasis in AGC.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plasminógeno
/
Neoplasias Gástricas
/
Activadores Plasminogénicos
/
Inhibidor 1 de Activador Plasminogénico
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Mucosa Gástrica
/
Ganglios Linfáticos
/
Metástasis de la Neoplasia
Límite:
Humanos
Idioma:
Inglés
Revista:
Cancer Research and Treatment
Año:
2015
Tipo del documento:
Artículo
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