Effect of p38-MAPK and STAT3 on biological properties of DPSCs under different oxidative stress / 中国免疫学杂志

ABSTRACT

Objective: To investigate the effect on the proliferation, mineralization and adipogenic differentiation of dental pulp stem cells ( DPSCs) under different oxidative stress by p38-MAPK and STAT3 signaling pathway. Methods: DPSCs was isolated and identified from pulp tissue by trypsin digestion. The CCK8 detection cell model was divided into oxygen group, 3％ O2 group, 6％ O2 group, 9％ O2 group firstly, each group included the SH-4-54 treatment group, and the SB203580 treatment group, a total of 12 groups. Cell proliferation was detected by CCK8 test for 6 h, 12 h, 24 h, 36 h, 48 h. We observed the differentiation of DPSCs mineralization by alizarin red staining involved in p38-MAPK and STAT3 signaling pathway, and the a-dipogenic differentiation of DPSCs by oil red O staining. Results: DPSCs were cultured by the enzyme digestion method, flow cytometry showed that DPSCs spec-ific marker Stro-1 expression was 96. 85％, CD146 positive expression was 90. 94％, the positive low expression of CD45 was 1. 02％, the positive low expression of CD34 was 40. 76％, p38-MAPK inhibited DPSCs with different oxygen environment. The proliferation of STAT3 promotes the survival of groups DPSCs, the p38-MAPK signaling pathway plays a positive role in the differentiation of mineralization of DPSCs and the STAT3 signaling pathway plays negative regulation role, the two signaling pathway play opposite effects in adipogenic differentiation research. Conclusion: The proliferation rate of DPSCs is higher in normoxic environment, and the STAT3 signal pathway can promote the proliferation of DPSCs, the MAPK signal pathway promotes the odontoblastic differentiation of DPSCs.