Shikonin suppresses invasion and migration of non-small cell lung cancer cells A549 / 中国免疫学杂志

ABSTRACT

Objective: Lung cancer is the most commonly diagnosed cancers and the leading cause of cancer death worldwide. This study aims to explore the effect and mechanism of shikonin on invasion and migration of non-small cell lung cancer cells A549. Methods: Cell viability was detected by CCK-8. Transwell assay was performed to analyze the invasion of A549. Wound healing assay was used to measure the migration of A549. The expression of vascular endothelial growth factor ( VEGF), matrix metalloprotein 14 ( MMP-14), fibronectin ( FN), Vimentin, PI3 K, p-AKT and p53 was tested by Western blot. Results: Cell viability of A549 was reduced by shikonin. Compared with control group, the invasion of A549 in shikonin group ( 20, 50, 100 mmol/L) was decreased as well as the migration ( P＜0. 05). The expression of VEGF, MMP-14, Fn and Vimentin in shikonin group ( 20, 50, 100 mmol/L) was lower than control group ( P＜0. 05). Compared with A549 group, the expression of PI3 K and p-AKT in IGF-1 group was increased with alleviated expression of p53 ( P＜0. 05). Compared with IGF-1 group, the expression of PI3 K and p-AKT in shikonin group ( 20, 50, 100 mmol/L) declined with enhancive expression of p53 ( P ＜ 0. 05). Conclusion: Shikonin suppresses invasion and migration of NSCLC cells A549 through inhibition of PI3 K/AKT signal pathway.