The study on growth and proliferation of neural stem cells from rats in vitro / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 747-750, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-746592
ABSTRACT
OBJECTIVE@#To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation.@*METHOD@#NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation.@*RESULT@#NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive.@*CONCLUSION@#NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Diferenciación Celular
/
Células Cultivadas
/
Embriología
/
Medio de Cultivo Libre de Suero
/
Ratas Sprague-Dawley
/
Biología Celular
/
Células Madre Multipotentes
/
Proliferación Celular
/
Embrión de Mamíferos
/
Células-Madre Neurales
Límite:
Animales
/
Embarazo
Idioma:
Chino
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Año:
2008
Tipo del documento:
Artículo
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