The influence of autophagy-related genes about X-Ray on nasopharyngeal carcinoma CNE2 and CNE2/DDP cells / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 547-551, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-747313
ABSTRACT
OBJECTIVE@#To study the relationship between the radiotherapy resistance and autophagy. To provide a theoretiacal basis for drugs that regulate autophagy to improve radiotherapy sensitivity.@*METHOD@#Flow cytometry (FCM) was performed to analyze the distribution of the cell cycle of CNE2 and CNE2/DDP cells under the action of X radiation. The expression of autopagy-specific gene Beclin1 and microtubule-associated protein light chain 3β (MAPLC3β) in CNE2 and CNE2/DDP cells was determined by real time PCR and Immumofluorescence staining.@*RESULT@#CNE2/DDP and their parental CNE2 cells produced the G2-M phase arrest under the action of X radiation. With the radiation dose increasing,The cells which in the G2-M phase were more and more (P<0. 05). The G2-M phase arrest in CNE2/DDP cells was more obvious than in CNE2 cells (P<0. 05). The expression of Beclin1 and MAPLC3β in CNE2 and CNE2/DDP cells increased under the action of X radiation. What's more, the raise was more and more obvious with the increase of the irradiation dose(P<0. 05). The expression levels of Beclin1 and MAPLC3β in CNE2/DDP was lower than that in CNE2 cells (P<0. 05).@*CONCLUSION@#Autophagic cell death may be the one manner of death in nasopharyngeal carcinoma CNE2 and CNE2/DDP cells under the action of X radiation. The radiation resistance of CNE2/DDP cells may be related to the low expression of autophagy-related genes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Efectos de la Radiación
/
Tolerancia a Radiación
/
Autofagia
/
Rayos X
/
Carcinoma
/
Ciclo Celular
/
Neoplasias Nasofaríngeas
/
Línea Celular Tumoral
/
Relación Dosis-Respuesta en la Radiación
/
Proteínas Reguladoras de la Apoptosis
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Año:
2015
Tipo del documento:
Artículo
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