Antisense oligonucleotides targeting XIAP induce apoptosis and enhance radiotherapeutic activity against hep-2 cells in vitro / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To investigate the down-regulation effect of antisense oligonucleotides (AS ODNs) targeting X-chromosome-linked inhibitors of apoptosis (XIAP) on hep-2 cells apoptosis and radiotherapy sensitivity in vitro.@*METHOD@#G4 AS ODN was transfected into cultured hep-2 cells which received radiation 6 hours later. Twenty-four hours after radiation, cells were observed under inverted phase contrast microscope and fluorescence microscope. Apoptosis rate, cell viability, expression of XIAP mRNA and protein were tested.@*RESULT@#In cultured hep-2 cells, G4 AS ODN down-regulated XIAP mRNA expression. Furthermore, the protein expression of XIAP and the cell viability decreased too. In contrast to that, the scrambled control ODN caused minor XIAP loss and less cell inhibition. In addition, G4 AS ODNs activated Hep-2 cells after the radiation of 4 Gy Co60 ray.@*CONCLUSION@#XIAP is a viable target for cancer therapy in human laryngeal neoplasms. In cultured Hep-2 cells, AS ODN targeting XIAP can down-regulate protein expression of XIAP, induce cell apoptosis and enhance the radiotherapy sensitivity.