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Effects of rosiglitazone on the Hep-2 cell proliferation, cell cycle and COX-2 expression / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery ; (24): 315-317, 2010.
Artículo en Chino | WPRIM | ID: wpr-748005
ABSTRACT
OBJECTIVE@#To explore the effect of rosiglitazone (ROS) on proliferation of human laryngeal carcinoma Hep-2 cells and it's mechanism.@*METHOD@#Methabenzthiazuron (MTT) was used to observe the proliferation of human laryngeal carcinoma Hep-2 cells by various concentrations of ROS at different times. Flow cytometry (FCM) used to measure the cell cycle and apoptosis rate. RT-PCR was used to measure the expression of cyclooxygenase-2 (COX-2) mRNA.@*RESULT@#The inhibited growth of ROS to Hep-2 cells in a dose-dependent and time-dependent manner (P < 0.01). The cell cycle was arrested in G0/G1 phase, which with a typical sub G1 peak, and the apoptosis rate increased in a time-dependent manner (P < 0.05). The expression of COX-2 mRNA in Hep-2 cells was significantly down-regulated by ROS (P < 0.01).@*CONCLUSION@#The function of growth inhibition and apoptosis induction of ROS on human laryngeal cancer Hep-2 cells was obvious, and its mechanism was related to block cell cycle at the G0/G1 phase and decrease the expression of COX-2.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Carcinoma de Células Escamosas / Ciclo Celular / Neoplasias Laríngeas / Apoptosis / Línea Celular Tumoral / Tiazolidinedionas / Proliferación Celular / Ciclooxigenasa 2 / Rosiglitazona Límite: Humanos Idioma: Chino Revista: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Año: 2010 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Carcinoma de Células Escamosas / Ciclo Celular / Neoplasias Laríngeas / Apoptosis / Línea Celular Tumoral / Tiazolidinedionas / Proliferación Celular / Ciclooxigenasa 2 / Rosiglitazona Límite: Humanos Idioma: Chino Revista: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Año: 2010 Tipo del documento: Artículo