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Construction and expression of the targeting super-antigen EGF-SEA fusion gene / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery ; (24): 638-656, 2014.
Artículo en Chino | WPRIM | ID: wpr-748160
ABSTRACT
OBJECTIVE@#To construct expression vector for the SEA-EGF fusion gene.@*METHOD@#Clone the SEA gene and the EGF gene segment with PCR and RT-PCR independently, and connect this two genes by the bridge PCR. Insert the fusion gene EGF-SEA into the expression vector PET-44. Induced the secretion of the fusion protein SEA-EGF by the antileptic.@*RESULT@#The gene fragment encoding EGF and SEA mature peptide was successfully cloned. The fusion gene EGF-SEA was successfully constructed and was inserted into expression vector.@*CONCLUSION@#The new recombinant expression vector for fusion gene EGF-SEA is specific for head and neck cancer, laid the foundation for the further study of fusion protein SEA-EGF targeting immune therapy in head and neck tumors.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Recombinantes de Fusión / Quimioterapia / Enterotoxinas / Factor de Crecimiento Epidérmico / Terapia Molecular Dirigida / Vectores Genéticos / Genética / Neoplasias de Cabeza y Cuello Límite: Humanos Idioma: Chino Revista: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Recombinantes de Fusión / Quimioterapia / Enterotoxinas / Factor de Crecimiento Epidérmico / Terapia Molecular Dirigida / Vectores Genéticos / Genética / Neoplasias de Cabeza y Cuello Límite: Humanos Idioma: Chino Revista: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Año: 2014 Tipo del documento: Artículo