Research of 10-23 DNAZyme inhibit the expression of eIF4E genes / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 552-554, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-748373
ABSTRACT
OBJECTIVE@#To explore the possibility of 10-23DNAzyme becoming a new gene therapy for laryngeal carcinoma treatment at the cell level.@*METHOD@#Thiosthorothioate 10-23DNAzyme specific to eIF4E gene mRNA 1059 was designed and synthesized, and its inhibition effects on the expression of eIF4E gene in Hep-2 cells were observed.@*RESULT@#The expression of eIF4E gene was remarkable depressed after Hep-2 cells was transfected by DNAzyme. The level of inhibiting eIF4E in hep-2 cells transfected by DNAzyme was lower than that by only lipofectamine 2000 transfected and Hep-2.@*CONCLUSION@#The expression of eIF4E gene in Hep-2 cells 10-23DNAzyme can be highly blocked. It is a specific and effective gene therapeutic means.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN de Cadena Simple
/
ARN Mensajero
/
Transfección
/
Terapia Genética
/
Expresión Génica
/
Neoplasias Laríngeas
/
Apoptosis
/
ADN Catalítico
/
Factor 4E Eucariótico de Iniciación
/
Línea Celular Tumoral
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Año:
2007
Tipo del documento:
Artículo
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