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Effect of clarithromycin on the expressions of cyclooxygenase-2 and nuclear factor-kappa B in nasal polyps in vitro / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery ; (24): 769-772, 2009.
Artículo en Chino | WPRIM | ID: wpr-748659
ABSTRACT
OBJECTIVE@#To detect the expression and correlation of cyclooxygenase-2 (COX-2) and nuclear factor-kappa B (NF-kappaB) in nasal polyps co-cultured with clarithromycin, and to investigate their roles in CRS pathogenesis.@*METHOD@#Nasal polyps from 11 patients with chronic rhinosinusitis (CRS) were cultured for 24 hours with different doses of clarithromycin (0, 10(-6), 10(-5), 10(-4) mol/L). Western blot and real time fluorescence quantitative PCR were performed to detect the expressions of COX-2 and NF-kappaB subunits.@*RESULT@#The expression levels of COX-2, NF-kappaBp50 and NF-kappaBp65 were most high in control groups (0 mol/L clarithromycin). The expressions of COX-2, NF-kappaBp50 and NF-kappaBp65 were dose-dependently attenuated as the concentrations of clarithromycin increased. Significantly positive correlation between RNA expressions of COX-2 and NF-kappaB subunits in each CRS group was confirmed by Pearson correlation treatment (P<0.05).@*CONCLUSION@#The increased expression of COX-2 is involved in the inflammation in CRS. It indicate that clarithromycin may play an anti-inflammatory effect on CRS by decreasing the synthesis of COX-2 through blocking NF-kappaB pathway.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Técnicas In Vitro / Pólipos Nasales / Claritromicina / Ciclooxigenasa 2 / Subunidad p50 de NF-kappa B / Factor de Transcripción ReIA / Metabolismo Límite: Adulto / Femenino / Humanos / Masculino Idioma: Chino Revista: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Técnicas In Vitro / Pólipos Nasales / Claritromicina / Ciclooxigenasa 2 / Subunidad p50 de NF-kappa B / Factor de Transcripción ReIA / Metabolismo Límite: Adulto / Femenino / Humanos / Masculino Idioma: Chino Revista: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Año: 2009 Tipo del documento: Artículo