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Inhibition of growth and proliferation of Hep-2 cells by targeting c-myc gene using small interference RNA technology / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery ; (24): 399-402, 2008.
Artículo en Chino | WPRIM | ID: wpr-749060
ABSTRACT
OBJECTIVE@#To investigate the effect of small interfering RNA (siRNA) targeting c-myc gene in Hep-2 cells.@*METHOD@#siRNA targeting c-myc mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by MTT, morphology, real time PCR assay.@*RESULT@#1) The MTT result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication; 2) The real time PCR result showed c-myc at mRNA level inhibition ratio at 94% in group S3; 3) The morphology result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication, the cell heteromorphism was diminished.@*CONCLUSION@#siRNA targeting c-myc can remarkably suppress the Hep-2 cell growth and multiplication.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Transfección / División Celular / Genes myc / Apoptosis / Marcación de Gen / ARN Interferente Pequeño / Línea Celular Tumoral / Proliferación Celular / Genética Límite: Humanos Idioma: Chino Revista: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Transfección / División Celular / Genes myc / Apoptosis / Marcación de Gen / ARN Interferente Pequeño / Línea Celular Tumoral / Proliferación Celular / Genética Límite: Humanos Idioma: Chino Revista: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Año: 2008 Tipo del documento: Artículo