Effect of Amniotic Membrane Extract on Cultured Human Nasal Mucosa Fibroblasts
Journal of the Korean Ophthalmological Society
;
: 1939-1944, 2015.
Artículo
en Coreano
| WPRIM
| ID: wpr-74924
ABSTRACT
PURPOSE:
To investigate the effects of amniotic membrane extract (AME) on the survival of cultured human nasal mucosa fibroblasts.METHODS:
Primary cultured human nasal mucosa fibroblasts were exposed to 0, 10, 20, or 30 microg/mL AME for 3 days. The survival of the human nasal mucosa fibroblasts was measured using the MTT assay and apoptosis was evaluated with flow cytometry using annexin-V/propidium iodide double staining.RESULTS:
AME decreased significantly in fibroblast proliferation after exposure to 10 microg/mL (p = 0.000), and caused significant apoptosis of the fibroblasts after exposure to 10 microg/mL (p = 0.024).CONCLUSIONS:
AME decreased fibroblast proliferation in vitro at least through induction of apoptosis. Therefore, adjuvant use of AME during endonasal dacryocystorhinostomy may improve clinical outcomes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Dacriocistorrinostomía
/
Apoptosis
/
Fibroblastos
/
Citometría de Flujo
/
Amnios
/
Mucosa Nasal
Límite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Año:
2015
Tipo del documento:
Artículo
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