Comparison of ELISA and pharmacia UNICAP detection reagent for house dust mite specific IgG4 / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
;
(24): 921-924, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-749263
ABSTRACT
OBJECTIVE@#To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic.@*METHOD@#The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated.@*RESULT@#The stability of ELISA reagent was good with low intra and inter assay variations (3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods.@*CONCLUSION@#The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sangre
/
Calibración
/
Inmunoglobulina G
/
Ensayo de Inmunoadsorción Enzimática
/
Pyroglyphidae
/
Alergia e Inmunología
/
Indicadores y Reactivos
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Año:
2013
Tipo del documento:
Artículo
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