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Anti-allergic effects of xuebijing and potential role of heme oxygenase-1 against ovalbumin-induced murine allergic rhinitis model / 临床耳鼻咽喉头颈外科杂志
Article en Zh | WPRIM | ID: wpr-749271
Biblioteca responsable: WPRO
ABSTRACT
OBJECTIVE@#In this study, we investigated the anti-inflammation effects of Xuebijing in OVA-induced murine allergic rhinitis model. Furthermore, we determined whether heme oxygenase (HO)-1 is required for the protective activity of Xuebijing.@*METHOD@#Airways of OVA-sensitized mice exposed to OVA challenge developed eosinophilia, mucus hypersecretion and increased cytokine levels. Levels of interleukin IL-4, IL-5, IL-13, and tumor necrosis factor (TNF)-alpha in nasal lavage fluid were measured using enzyme-linked immunosorbent assays (ELISAs). Lung tissue and nasal mucosa sections were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration and mucus production, Immunohistochemistry, Real-time PCR and Western Blot analyses for HO-1 protein expression.@*RESULT@#Orally administered Xuebijing significantly inhibited the number of OVA-induced inflammatory cells and IgE production, along with reduced T-helper (Th) 2 cytokine levels, such as IL-4, IL-5 and IL-13, improved the level of IFN-gamma, in nasal lavage fluid. In addition, Xuebijing induced a marked decrease in OVA-induced inflammatory cell infiltration and mucus production in nasal and lung tissues. These effects were correlated with HO-1 mRNA and protein induction.@*CONCLUSION@#Our results indicate that Xuebijing protects against OVA-induced airway inflammation, at least in part, via HO-1 upregulation.
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Farmacología / Inmunoglobulina E / Medicamentos Herbarios Chinos / Rinitis Alérgica Perenne / Interleucina-4 / Interleucina-5 / Factor de Necrosis Tumoral alfa / Interleucina-13 / Usos Terapéuticos / Quimioterapia Tipo de estudio: Prognostic_studies Límite: Animals Idioma: Zh Revista: Lin chuang er bi yan hou ke za zhi Año: 2013 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Farmacología / Inmunoglobulina E / Medicamentos Herbarios Chinos / Rinitis Alérgica Perenne / Interleucina-4 / Interleucina-5 / Factor de Necrosis Tumoral alfa / Interleucina-13 / Usos Terapéuticos / Quimioterapia Tipo de estudio: Prognostic_studies Límite: Animals Idioma: Zh Revista: Lin chuang er bi yan hou ke za zhi Año: 2013 Tipo del documento: Article