Murine subcutaneous immunotherapy models with beneficial immunological and physiological effects
Asia Pacific Allergy
;
(4): 50-58, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-749928
ABSTRACT
BACKGROUND:
Immunotherapy was introduced 100 years ago and has a unique role in the treatment of allergic diseases in that only immunotherapy can induce long-term immunological tolerance. However, only a few mouse models of immunotherapy have been developed so far.OBJECTIVE:
We tried to establish murine immunotherapy models that have similar findings in human using subcutaneous rush immunotherapy-like schedule.METHODS:
To determine the maximal safe or maximal tolerable dose, injection dose was doubled twice a day from the dose of sensitization. Mice with established asthma using ovalbumin (OVA) were repeatedly injected with OVA from the dose of sensitization subcutaneously twice a day after reaching to the maximal safe or maximal tolerable dose, mice were injected with each dose either 10 times or 24 times.RESULTS:
Short term immunotherapy (10 times) with the maximal safe and tolerable dose of OVA showed decreased IL-5 production, decreased IL-5/INF-γ ratio, and increased IgG2a/IgG1 but there was no significant difference in airway hyperresponsiveness (AHR) or airway inflammation. Prolonged immunotherapy (24 times) with the maximal tolerable dose not only decreased cytokine productions of IL-5 and even INF-γ, but also decreased IgE, IgG1 and even IgG2a production. Remarkably, the prolonged immunotherapy provided a protective effect on AHR.CONCLUSION:
This study suggested immunotherapy models with some beneficial immunological and physiological effects in murine asthma.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Óvulo
/
Citas y Horarios
/
Asma
/
Inmunoglobulina E
/
Inmunoglobulina G
/
Ovalbúmina
/
Interleucina-5
/
Modelos Animales
/
Hipersensibilidad
/
Inmunoterapia
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Asia Pacific Allergy
Año:
2013
Tipo del documento:
Artículo
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