Expression and clinic significance of long non-coding RNA ZEB1-AS1 in breast cancer / 国际肿瘤学杂志

ABSTRACT

Objective To detect the expression and clinic significance of long non-coding RNA ZEB1-AS1 in breast cancer. Methods A total of 130 patients with breast cancer in Baoji Central Hospital of Shaanxi Province from June 2007 to April 2015 were selected. Quantitative real-time PCR(qRT-PCR)was used to detect the expression level of ZEB1-AS1 in breast cancer tissues and corresponding normal tissues,and the rela-tionships between the expression level of ZEB1-AS1 and the clinic characteristics of the patients and their overall survival time were analyzed. siRNA was used to disturb the expression of ZEB1-AS1. CCK-8 assay, clone formation assay and Transwell assay were used to detect the proliferation,cloning ability and migration of breast cancer MCF-7 cells in control group,siRNA-1 group and siRNA-2 group. Results The expression level of ZEB1-AS1 in breast cancer tissues was higher than that in corresponding normal tissues[M(QR )0. 0016 (0. 0051)vs. 0. 0009(0. 0015);Z = - 4. 426,P < 0. 001]. The higher expression of ZEB1-AS1 was correlated with lymphatic metastasis(χ2 = 9. 148,P = 0. 027),negative human epidermal growth factor recep-tor 2(χ2 = 5. 039,P = 0. 025),triple negative breast cancer(χ2 = 4. 597,P = 0. 032). The patients with the higher expression of ZEB1-AS1 had a shorter overall survival time compared with the patients with the lower expression of ZEB1-AS1(χ2 = 14. 340,P < 0. 001). CCK-8 assay showed that knock down of ZEB1-AS1 after 72 h,the absorbance values of the control group,siRNA-1 group and siRNA-2 group were 0. 605 ± 0. 049, 0. 488 ± 0. 054,0. 417 ± 0. 038 respectively,with a statistically significant different( F = 15. 936,P <0. 001),and the two siRNA groups were significantly inhibited in cell proliferation compared with the control group(both P < 0. 05). The colonies of the control group,siRNA-1 group and siRNA-2 group were 297. 5 ± 11. 4,192. 0 ± 12. 1,204. 8 ± 12. 8 respectively,with a statistically significant different(F = 112. 526,P <0. 001),and the two siRNA groups were significantly inhibited in the cell clone compared with the control group(both P < 0. 001). The migratory cells numbers of the control group,siRNA-1 group and siRNA-2 group were 184. 5 ± 8. 6,147. 5 ± 18. 6,57. 6 ± 7. 3 respectively,with a statistically significant different( F =12. 409,P = 0. 001),and the two siRNA groups were significantly inhibited in the cell migration(both P <0. 001). Conclusion ZEB1-AS1 is overexpressed in breast cancer,overexpression of ZEB1-AS1 induces a shorter overall survival in breast cancer patients,and knock down of ZEB1-AS1 can inhibit the proliferation, migration and colony formation ability of the breast cancer cell line.