Role of Nitric Oxide on the Proliferation of Human Tenon Capsule Fibroblasts
Journal of the Korean Ophthalmological Society
;
: 1670-1674, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-7544
ABSTRACT
PURPOSE:
To investigate the role of nitric oxide(NO) on the survival and proliferation of human Tenon capsule fibroblasts(HTCF) in tissue culture.METHODS:
Following exposure to NO donor such as sodium nitroprusside(SNP) or to NO synthase inhibitor such as N omega-Nitro-L-arginine methyl ester(L-NAME) at various concentrations for 24 hr in the media with or without serum, the cellular proliferation and nitrite production were assessed by rapid colorimetric assay (MTT assay) and Griess assay, respectively.RESULTS:
L-NAME decreased the cellular proliferation of HTCF in a dose-dependent manner. In contrast, SNP increased the proliferation in a dose-dependent manner. The differences of nitrite production were more remarkable in the serum-deprived condition with the L-NAME or SNP administration.CONCLUSIONS:
NO donor has a proproliferative, and NOS inhibitor has a antiproliferative activities on the proliferation of HTCF. This suggests that NO may be act as an important modulator in wound healing.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sodio
/
Donantes de Tejidos
/
Cicatrización de Heridas
/
Nitroprusiato
/
Óxido Nítrico Sintasa
/
NG-Nitroarginina Metil Éster
/
Nitroarginina
/
Proliferación Celular
/
Cápsula de Tenon
/
Fibroblastos
Límite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Año:
2003
Tipo del documento:
Artículo
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