Overexpression of sigma-1 receptor inhibits ADAM10 and ADAM17 mediated shedding in vitro
Protein & Cell
;
(12): 153-159, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-757300
ABSTRACT
The sigma-1 receptor is a molecular chaperone protein highly enriched in the brain. Recent studies linked it to many diseases, such as drug addition, Alzheimer's disease, stroke, depression, and even cancer. Sigma-1 receptor is enriched in lipid rafts, which are membrane microdomains essential in signaling processes. One of those signaling processes is ADAM17- and ADAM10-dependent ectodomain shedding. By using an alkaline phosphatase tagged substrate reporter system, we have shown that ADAM10-dependent BTC shedding was very sensitive to both membrane lipid component change and sigma-1 receptor agonist DHEAS treatment while ADAM17-dependent HB-EGF shedding was not; and overexpression of sigma-1 receptor diminished ADAM17- and ADAM10-dependent shedding. Our results indicate that sigma-1 receptor plays an important role in modifying the function of transmembrane proteases.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Expresión Génica
/
Chlorocebus aethiops
/
Receptores sigma
/
Células COS
/
Microdominios de Membrana
/
Péptidos y Proteínas de Señalización Intercelular
/
Proteínas ADAM
/
Secretasas de la Proteína Precursora del Amiloide
/
Células HEK293
/
Betacelulina
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Protein & Cell
Año:
2012
Tipo del documento:
Artículo
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