Analysis of the p53/CEP-1 regulated non-coding transcriptome in C. elegans by an NSR-seq strategy
Protein & Cell
;
(12): 770-782, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-757473
ABSTRACT
In recent years, large numbers of non-coding RNAs (ncRNAs) have been identified in C. elegans but their functions are still not well studied. In C. elegans, CEP-1 is the sole homolog of the p53 family of genes. In order to obtain transcription profiles of ncRNAs regulated by CEP-1 under normal and UV stressed conditions, we applied the 'not-so-random' hexamers priming strategy to RNA sequencing in C. elegans, This NSR-seq strategy efficiently depleted rRNA transcripts from the samples and showed high technical replicability. We identified more than 1,000 ncRNAs whose apparent expression was repressed by CEP-1, while around 200 were activated. Around 40% of the CEP-1 activated ncRNAs promoters contain a putative CEP-1-binding site. CEP-1 regulated ncRNAs were frequently clustered and concentrated on the X chromosome. These results indicate that numerous ncRNAs are involved in CEP-1 transcriptional network and that these are especially enriched on the X chromosome in C. elegans.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Efectos de la Radiación
/
Rayos Ultravioleta
/
Cromosoma X
/
Sitios de Unión
/
Proteína p53 Supresora de Tumor
/
Regiones Promotoras Genéticas
/
Análisis de Secuencia de ARN
/
Caenorhabditis elegans
/
ARN no Traducido
/
Proteínas de Caenorhabditis elegans
Límite:
Animales
Idioma:
Inglés
Revista:
Protein & Cell
Año:
2014
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS