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A new unconventional HLA-A2-restricted epitope from HBV core protein elicits antiviral cytotoxic T lymphocytes
Protein & Cell ; (12): 317-327, 2014.
Artículo en Inglés | WPRIM | ID: wpr-757497
ABSTRACT
Cytotoxic T cells (CTLs) play a key role in the control of Hepatitis B virus (HBV) infection and viral clearance. However, most of identified CTL epitopes are derived from HBV of genotypes A and D, and few have been defined in virus of genotypes B and C which are more prevalent in Asia. As HBV core protein (HBc) is the most conservative and immunogenic component, in this study we used an overlapping 9-mer peptide pool covering HBc to screen and identify specific CTL epitopes. An unconventional HLA-A2-restricted epitope HBc141-149 was discovered and structurally characterized by crystallization analysis. The immunogenicity and anti-HBV activity were further determined in HBV and HLA-A2 transgenic mice. Finally, we show that mutations in HBc141-149 epitope are associated with viral parameters and disease progression in HBV infected patients. Our data therefore provide insights into the structure characteristics of this unconventional epitope binding to MHC-I molecules, as well as epitope specific CTL activity that orchestrate T cell response and immune evasion in HBV infected patients.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Unión Proteica / Sitios de Unión / Ratones Transgénicos / Linfocitos T Citotóxicos / Antígeno HLA-A2 / Química / Virus de la Hepatitis B / Secuencia de Aminoácidos / Estructura Terciaria de Proteína / Alergia e Inmunología Tipo de estudio: Estudio pronóstico Límite: Animales / Femenino / Humanos / Masculino Idioma: Inglés Revista: Protein & Cell Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Unión Proteica / Sitios de Unión / Ratones Transgénicos / Linfocitos T Citotóxicos / Antígeno HLA-A2 / Química / Virus de la Hepatitis B / Secuencia de Aminoácidos / Estructura Terciaria de Proteína / Alergia e Inmunología Tipo de estudio: Estudio pronóstico Límite: Animales / Femenino / Humanos / Masculino Idioma: Inglés Revista: Protein & Cell Año: 2014 Tipo del documento: Artículo