CD1d(hi)CD5⁺ B cells differentiate into antibody-secreting cells under the stimulation with calreticulin fragment
Protein & Cell
; (12): 872-881, 2013.
Article
en En
| WPRIM
| ID: wpr-757545
Biblioteca responsable:
WPRO
ABSTRACT
Calreticulin (CRT) is a multifunctional molecule in both intracellular and extracellular environment. We have previously found that a recombinant CRT fragment (rCRT/39-272) could modulate T cell-mediated immunity in mice via activation and expansion of CD1d(hi)CD5⁺ B cells as well as induction of CRT-specific regulatory antibodies. Antibody secreting cells (ASCs) are terminally differentiated B cells responsible for producing antibodies to participate in positive immune response as well as immune regulation. In this study, we demonstrate that rCRT/39-272 differentiates murine CD1d(hi)CD5⁺ B cells into ASCs marked by increased expression of plasma cell-associated transcription factors and production of polyreactive antibodies against DNA and CRT in vitro. Intraperitoneal administration of rCRT/39-272 augmented differentiation of CD1d(hi)CD5⁺ B cells into ASCs in naïve mice or mice with experimental autoimmune encephalomyelitis. Thus, we propose that ASC differentiation and subsequent antibody production of CD1d(hi)CD5⁺ B cells are key steps in CRT-mediated immunoregulation on inflammatory T cell responses.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Fragmentos de Péptidos
/
Farmacología
/
Autoanticuerpos
/
Solubilidad
/
Linfocitos B
/
Diferenciación Celular
/
Química
/
Antígenos CD5
/
Biología Celular
/
Calreticulina
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Protein & Cell
Año:
2013
Tipo del documento:
Article