MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1
Protein & Cell
; (12): 851-861, 2014.
Article
en En
| WPRIM
| ID: wpr-757640
Biblioteca responsable:
WPRO
ABSTRACT
MicroRNAs (miRNAs) that exert function by posttranscriptional suppression have recently brought insight in our understanding of the role of non-protein-coding RNAs in carcinogenesis and metastasis. In this study, we described the function and molecular mechanism of miR-139-5p in colorectal cancer (CRC) and its potential clinical application in CRC. We found that miR-139-5p was significantly downregulated in 73.8% CRC samples compared with adjacent noncancerous tissues (NCTs), and decreased miR-139-5p was associated with poor prognosis. Functional analyses demonstrated that ectopic expression of miR-139-5p suppressed CRC cell migration and invasion in vitro and metastasis in vivo. Mechanistic investigations revealed that miR-139-5p suppress CRC cell invasion and metastasis by targeting AMFR and NOTCH1. Knockdown of the two genes phenocopied the inhibitory effect of miR-139-5p on CRC metastasis. Furthermore, the protein levels of the two genes were upregulated in CRC samples compared with NCTs, and inversely correlated with the miR-139-5p expression. Increased NOTCH1 protein expression was correlated with poor prognosis of CRC patients. Together, our data indicate that miR-139-5p is a potential tumor suppressor and prognostic factor for CRC, and targeting miR-139-5p may repress the metastasis of CRC and improve survival.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Patología
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Terapéutica
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Secuencia de Bases
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Homología de Secuencia de Ácido Nucleico
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Neoplasias Colorrectales
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Regulación hacia Abajo
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Regulación Neoplásica de la Expresión Génica
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Análisis de Supervivencia
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Movimiento Celular
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Protein & Cell
Año:
2014
Tipo del documento:
Article