Twenty years hunting for sulfur in DNA
Protein & Cell
;
(12): 14-21, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-757710
ABSTRACT
Here we tell a 20-year long story. It began with an easily overlooked DNA degradation (Dnd) phenomenon during electrophoresis and eventually led to the discovery of an unprecedented DNA sulfur modification governed by five dnd genes. This unusual DNA modification, called phosphorothioation, is the first physiological modification identified on the DNA backbone, in which the nonbridging oxygen is replaced by sulfur in a sequence selective and stereo-specific manner. Homologous dnd gene clusters have been identified in diverse and distantly related bacteria and thus have drawn immediate attention of the entire microbial scientific community. Here, we summarize the progress in chemical, genetic, enzymatic, bioinformatical and analytical aspects of this novel postreplicative DNA modification. We also discuss perspectives on the physiological functions of the DNA phosphorothioate modification in bacteria and their implications.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Azufre
/
Bacterias
/
ADN Bacteriano
/
Química
/
Familia de Multigenes
/
Streptomyces lividans
/
Historia del Siglo XX
/
Historia del Siglo XXI
/
Genes Bacterianos
/
Genética
Tipo de estudio:
Estudio pronóstico
Idioma:
Inglés
Revista:
Protein & Cell
Año:
2010
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS