AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth
Protein & Cell
;
(12): 155-161, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-757826
ABSTRACT
Down syndrome cell adhesion molecule (DSCAM) acts as a netrin-1 receptor and mediates attractive response of axons to netrin-1 in neural development. However, the signaling mechanisms of netrin-DSCAM remain unclear. Here we report that AMP-activated protein kinase (AMPK) interacts with DSCAM through its γ subunit, but does not interact with DCC (deleted in colorectal cancer), another major receptor for netrin-1. Netrin-treatment of cultured cortical neurons leads to increased phosphorylation of AMPK. Both AMPK mutant with dominant-negative effect and AMPK inhibitor can significantly suppress netrin-1 induced neurite outgrowth. Together, these findings demonstrate that AMPK interacts with DSCAM and plays an important role in netrin-1 induced neurite outgrowth. Our study uncovers a previously unknown component, AMPK, in netrin-DSCAM signaling pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fosforilación
/
Fisiología
/
Unión Proteica
/
Proteínas Recombinantes de Fusión
/
Transfección
/
Transducción de Señal
/
Moléculas de Adhesión Celular
/
Células Cultivadas
/
Neuritas
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Protein & Cell
Año:
2013
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS